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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Related Experiment Video

Updated: Apr 6, 2026

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
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Acoustic Sample Deposition MALDI-MS (ASD-MALDI-MS): A Novel Process Flow for Quality Control Screening of Compound

Jefferson Chin1, Elizabeth Wood1, Grace S Peters2

  • 1Bristol-Myers Squibb Company, Research & Development, Disease Sciences and Biologics, Leads Discovery and Optimization, Wallingford, CT, USA.

Journal of Laboratory Automation
|July 24, 2015
PubMed
Summary

This study introduces a new quality control method for drug discovery screening. Acoustic sample deposition with mass spectrometry (ASD-MALDI-MS) rapidly analyzes small molecule libraries with minimal sample usage.

Keywords:
MALDI-MSacoustic sample depositionhigh-throughput screening

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Area of Science:

  • Drug Discovery and Development
  • Analytical Chemistry
  • Biotechnology

Background:

  • High-throughput screening (HTS) is vital for identifying drug leads.
  • Ensuring compound library quality is critical for HTS efficiency.
  • Limited sample amounts and large library sizes present significant QC challenges.

Purpose of the Study:

  • To develop a novel, low-sample-consumption process for quality control screening of small-molecule compound libraries.
  • To enable rapid, compound-specific molecular data generation for quality control.
  • To address the challenges of HTS compound library QC.

Main Methods:

  • A proof-of-concept study utilizing acoustic sample deposition (ASD) for offline sample preparation.
  • Nanoliter volumes of compounds deposited in an array format onto microscope slides.
  • Matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis of deposited samples.

Main Results:

  • Successful implementation of the ASD-MALDI-MS workflow for compound library QC.
  • Achieved a 75% first-pass positive identification rate in an initial 384-compound array study.
  • Demonstrated rapid analysis with an average time of less than 1 second per sample.

Conclusions:

  • The ASD-MALDI-MS workflow offers a highly efficient method for small-molecule compound library quality control.
  • This novel process significantly reduces sample consumption while providing valuable molecular data.
  • The method shows promise for improving the efficiency and success rate of early-stage drug discovery HTS campaigns.