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The Oncogenic Response to MiR-335 Is Associated with Cell Surface Expression of Membrane-Type 1 Matrix

Fausto Rojas1, Maria E Hernandez1, Milagros Silva1

  • 1Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America.

Plos One
|July 24, 2015
PubMed

Insights

MicroRNA miR-335 impacts cell behavior differently based on cell type. Its effects on cell proliferation and migration are linked to the expression of MT1-MMP, a proteinase involved in tumor progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • MicroRNA miR-335 exhibits dual roles as a tumor suppressor and oncogene.
  • MT1-MMP proteinase is frequently found in tumors and influences cell proliferation and migration.

Purpose of the Study:

  • To investigate the influence of miR-335 on MT1-MMP expression across different cell types.
  • To determine how miR-335 affects cell proliferation and migration in relation to MT1-MMP activity.

Main Methods:

  • Examined miR-335 effects on MT1-MMP cell surface expression in fibrosarcoma, prostate, breast, and glioblastoma cell lines.
  • Utilized in vitro wound healing assays to assess cell proliferation and migration.

Main Results:

  • miR-335 increased MT1-MMP expression in fibrosarcoma HT-1080 and benign prostate BPH-1 cells.
  • miR-335 enhanced proliferation and migration in HT-1080, BPH-1, and U87 glioblastoma cells, correlating with MT1-MMP expression.
  • No significant effect on proliferation or migration was observed in cells lacking prominent MT1-MMP activity.

Conclusions:

  • Cellular responses to miR-335 vary depending on cell type and MT1-MMP regulation.
  • MT1-MMP expression is a key factor mediating miR-335's impact on cell proliferation and migration.

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