[Hormonal Induction of In Vitro Maturation and Ovulation of Loach Oocytes and Obtaining Egg Cells Capable of...

Loach oocytes that have reached a definitive size and are surrounded by follicular envelopes are capable of maturation and ovulating under the effect of 1 μg/mL progesterone in 75% Leibovitz medium with 1 g/L sodium bicarbonate or with pH adjustment to 9.0 by 1 N sodium hydroxide. Inseminated eggs are developed until the stage of when adding 20% bovine serum to the incubation medium. Substitution of the bovine serum with 10-20% loach ovarian fluid or 20% carp ovarian fluid provides more complete development of inseminated eggs until the stage of prolarvae that pass to active nutrition.