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Updated: Apr 6, 2026
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Measurements of morphology and refractive indexes on human downy hairs using three-dimensional quantitative phase
SangYun Lee1, Kyoohyun Kim1, Yuhyun Lee2
1Korea Advanced Institute of Science and Technology, Department of Physics, 291 Daehak-Ro Yusung-Gu, Daejeon 305-701, Republic of Korea.
Abstract:
We present optical measurements of morphology and refractive indexes (RIs) of human downy arm hairs using three-dimensional (3-D) quantitative phase imaging techniques. 3-D RI tomograms and high-resolution two-dimensional synthetic aperture images of individual downy arm hairs were measured using a Mach–Zehnder laser interferometric microscopy equipped with a two-axis galvanometer mirror. From the measured quantitative images, the RIs and morphological parameters of downy hairs were noninvasively quantified including the mean RI, volume, cylinder, and effective radius of individual hairs. In addition, the effects of hydrogen peroxide on individual downy hairs were investigated.
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