Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Depressive Disorders: MDD and Dysthymia01:27

Depressive Disorders: MDD and Dysthymia

1.1K
Depressive disorders are a group of mental health conditions characterized by pervasive feelings of sadness, diminished pleasure in life, and a significant impact on daily functioning. These conditions are most prevalent in individuals during their 30s and affect women at twice the rate of men. Contrary to popular belief, younger individuals are generally more susceptible to these disorders than older adults. Two key types of depressive disorders include Major Depressive Disorder (MDD) and...
1.1K
Depressive Disorders: Etiology01:27

Depressive Disorders: Etiology

863
Depressive disorders result from a complex interplay of biological, psychological, and sociocultural factors, each contributing uniquely to the development and persistence of the condition. Understanding these factors provides critical insight into the multifaceted nature of depression.
Biological Factors in Depression
Biological predispositions significantly influence the risk of developing depressive disorders. Genetic studies highlight the role of variations in the serotonin transporter...
863
DNA Microarrays02:34

DNA Microarrays

23.0K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A pH-sensitive CuHP composite hydrogel featuring antibacterial, antioxidant and osteogenic properties for treating diabetic periodontitis.

Regenerative biomaterials·2025
Same author

Aptamer-Based Molecule-Responsive SERS System for Highly Efficient Detection of Cocaine.

Analytical chemistry·2025
Same author

Letter by Liu et al Regarding Article, "Rivaroxaban for 18 Months Versus 6 Months in Patients With Cancer and Acute Low-Risk Pulmonary Embolism: An Open-Label, Multicenter, Randomized Clinical Trial (ONCO PE Trial)".

Circulation·2025
Same author

Pharmacovigilance study of ramucirumab: A safety analysis based on the FDA adverse event reporting system.

Journal of cancer research and therapeutics·2025
Same author

Cleavage of N-terminus of polycystin-1 increases calcium permeability of polycystin-1/2 receptor channel complexes.

JCI insight·2025
Same author

Integrated Multi-Omics Investigation of Gypenosides' Mechanisms in Lowering Hepatic Cholesterol.

Biomolecules·2025

Related Experiment Video

Updated: Apr 6, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

16.0K

Microarray Analysis of the Major Depressive Disorder mRNA Profile Data.

Lishu Gao1, Yue Gao2, Enping Xu3

  • 1Department of Clinical Psychology, Hangzhou First People's Hospital, Hangzhou, Zhejiang, PR China.

Psychiatry Investigation
|July 25, 2015
PubMed
Summary

This study identified differentially expressed genes (DEGs) in major depressive disorder (MDD) brain tissue. Key genes in the striatum, prefrontal cortex, and hippocampus may offer new therapeutic targets for MDD.

Keywords:
Differentially expressed genesFunction enrichmentMajor depressive disorderPathway enrichment

More Related Videos

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

20.6K
Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
09:29

Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods

Published on: August 4, 2022

2.9K

Related Experiment Videos

Last Updated: Apr 6, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

16.0K
Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
16:13

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

20.6K
Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
09:29

Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods

Published on: August 4, 2022

2.9K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Major depressive disorder (MDD) is a prevalent mood disorder with significant psychophysiological alterations.
  • Understanding the genetic mechanisms underlying MDD is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the genetic underpinnings of MDD using gene expression profiling.
  • To identify differentially expressed genes (DEGs) and their associated pathways in MDD.

Main Methods:

  • Downloaded mRNA expression profile (GSE53987) from the Gene Expression Omnibus database.
  • Analyzed 105 post-mortem brain tissue samples (MDD vs. controls) from hippocampus, prefrontal cortex, and striatum.
  • Identified DEGs using Limma, enriched functions/pathways with DAVID, and constructed protein-protein interaction networks with Cytoscape.

Main Results:

  • Identified 241 DEGs in the hippocampus, 218 in the prefrontal cortex, and 327 in the striatum.
  • Enriched biological processes included glycan biosynthesis, RIG-I-like receptor signaling, and pyrimidine metabolism.
  • Key DEGs such as PTK2, AR, and IRAK1 were identified in specific brain regions.

Conclusions:

  • The striatum appears significantly affected in MDD.
  • Identified DEGs like PTK2, GALNT2, AR, IRAK1, and IL12A may serve as novel therapeutic targets for MDD treatment.