Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

End to end AI system for surgical gesture sequence recognition and clinical outcome prediction.

NPJ digital medicine·2026

Same author

Tumor anatomy predictors of mic and trifecta achievement in robot-assisted partial nephrectomy: a multicenter analysis stratified by PADUA score.

Journal of robotic surgery·2026

Same author

Single-trial assessment of gamma-frequency brain oscillations and their modulation using transcranial alternating current stimulation (tACS).

Brain stimulation·2026

Same author

[Validation of a new portable measurement method for home use-a feasibility study].

Urologie (Heidelberg, Germany)·2026

Same author

AI model predicts patient outcomes from surgical gestures and provides insights into explainability.

npj digital surgery·2026

Same author

Hemorrhage in Robot-assisted Partial Nephrectomy: A Multicentric, Retrospective Analysis of Blood Transfusion and Bleeding Requiring Intervention in 3726 Cases.

European urology open science·2026

Same journal

Erratum: Spectroscopy and Ground-State Transfer of Ultracold Bosonic ^{39}K^{133}Cs Molecules [Phys. Rev. Lett. 135, 203401 (2025)].

Physical review letters·2026

Same journal

Erratum: Lifetime of the ^{2}F_{7/2} Level in Yb^{+} for Spontaneous Emission of Electric Octupole Radiation [Phys. Rev. Lett. 127, 213001 (2021)].

Physical review letters·2026

Same journal

Laser-Plasma Based Seeded Free Electron Laser in the High-Gain Regime.

Physical review letters·2026

Same journal

Parent Hamiltonians for Stabilizer Quantum Many-Body Scars.

Physical review letters·2026

Same journal

Properties of Heavy Cosmic Nuclei Phosphorus, Chlorine, Argon, Potassium, and Calcium: Results from the Alpha Magnetic Spectrometer.

Physical review letters·2026

Same journal

Role of Spin-Isospin Symmetries in Nuclear β-Decays.

Physical review letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 6, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Scanning Quantum Dot Microscopy.

Christian Wagner^1,2, Matthew F B Green^1,2, Philipp Leinen^1,2

¹Peter Grünberg Institut (PGI-3), Forschungszentrum Jülich, 52425 Jülich, Germany.

Physical Review Letters

|July 25, 2015

Summary

This summary is machine-generated.

We developed a new scanning probe method for 3D electrostatic imaging with subnanometer resolution. This technique images molecular quadrupole and adatom dipole fields, enabling analysis of rough surfaces.

More Related Videos

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Related Experiment Videos

Last Updated: Apr 6, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Area of Science:

Surface science
Scanning probe microscopy
Quantum dots

Background:

Accurate characterization of electrostatic fields is crucial for understanding molecular and atomic interactions.
Existing techniques often lack the resolution or sensitivity required for nanoscale electrostatic mapping.

Purpose of the Study:

To introduce a novel scanning probe technique for high-resolution 3D imaging of local electrostatic potential fields.
To demonstrate the capability of imaging both molecular quadrupole and atomic dipole fields.
To showcase the technique's potential for analyzing samples with surface roughness.

Main Methods:

Utilizing an atomic force microscope (AFM) with a qPlus tuning fork operated at 5 K.
Attaching a molecular quantum dot to the AFM tip to register single electron charging events.
Analyzing the electrostatic fields generated by single molecules and adatoms.

Main Results:

Achieved subnanometer resolution in 3D electrostatic potential imaging.
Successfully imaged the quadrupole field of a single molecule.
Quantitatively measured the dipole field of a single metal adatom on a metal surface.
Demonstrated probing of electrostatic potentials at large distances, suitable for rough surfaces.

Conclusions:

The developed scanning probe technique offers unprecedented 3D electrostatic imaging capabilities at the nanoscale.
High sensitivity allows for detailed analysis of electrostatic interactions on complex surfaces.
This method opens new avenues for surface science and nanoscale characterization.