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Published on: July 8, 2025
In Silico Analysis of Tumor Necrosis Factor α-Induced Protein 8-Like-1 (TIPE1) Protein
Pei Shen1, Hong Zhang2, Zhaoliang Su3
1Department of Immunology, Institute of Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China; Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
Abstract:
Tumor necrosis factor α-induced protein 8 (TNFAIP8)-like protein 1 (TIPE1) was a member of TNFAIP8 family. Previous studies have shown that TIPE1 could induce apoptosis in hepatocellular carcinoma. In this study, we attempted to predict its potential structure. Bioinformatic analysis of TIPE1 was performed to predict its potential structure using the bioinfomatic web services or softwares. The results showed that the amino acid sequences of TIPE1 were well conserved in mammals. No signal peptide and no transmembrane domain existed in human TIPE1. The aliphatic index of TIPE1 was 100.75 and the theoretical pI was 9.57. TIPE1 was a kind of stable protein and its grand average of hydropathicity was -0.108. Various post-translational modifications were also speculated to exist in TIPE1. In addition, the results of Swiss-Model Server and Swiss-Pdb Viewer program revealed that the predicted three-dimensional structure of TIPE1 protein was stable and it may accord with the rule of stereochemistry. TIPE1 was predicted to interact with FBXW5, caspase8 and so on. In conclusion, TIPE1 may be a stable protein with no signal peptide and no transmembrane domain. The bioinformatic analysis of TIPE1 will provide the basis for the further study on the function of TIPE1.
Insights
Tumor necrosis factor α-induced protein 8 (TNFAIP8)-like protein 1 (TIPE1) is a stable protein. Bioinformatic analysis reveals TIPE1’s conserved structure and potential interactions, providing a basis for further functional studies.
Area of Science:
- Biochemistry
- Structural Biology
- Bioinformatics
Background:
- Tumor necrosis factor α-induced protein 8 (TNFAIP8)-like protein 1 (TIPE1) is part of the TNFAIP8 family.
- Previous research indicates TIPE1 can induce apoptosis in hepatocellular carcinoma.
Purpose of the Study:
- To predict the potential structure and properties of TIPE1 using bioinformatic tools.
- To establish a foundation for future research into TIPE1's biological functions.
Main Methods:
- Bioinformatic analysis of TIPE1 amino acid sequences.
- Utilizing web services and software for structural prediction.
- Employing Swiss-Model Server and Swiss-Pdb Viewer for 3D structure analysis.
Main Results:
- TIPE1 amino acid sequences are conserved across mammals.
- Human TIPE1 lacks signal peptides and transmembrane domains.
- TIPE1 is predicted to be a stable protein with a theoretical pI of 9.57 and an aliphatic index of 100.75.
- Predicted interactions include FBXW5 and caspase8.
- The 3D structure is predicted to be stable and stereochemically sound.
Conclusions:
- TIPE1 is a stable protein lacking signal peptides and transmembrane domains.
- Bioinformatic analysis provides crucial insights into TIPE1's structure and stability.
- This study lays the groundwork for investigating TIPE1's role in biological processes.
