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Related Experiment Video

Updated: Apr 6, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

2D map projections for visualization and quantitative analysis of 3D fluorescence micrographs.

G Hernán Sendra¹, Christian H Hoerth¹, Christian Wunder²

¹Center of Molecular Biology, University of Heidelberg (ZMBH), Heidelberg, Germany.

Scientific Reports

|July 25, 2015

Summary

Map3-2D software projects complex five-dimensional fluorescence microscopy images into 2D maps for clear visualization and analysis. This tool aids in studying cellular structures and dynamic processes on various surfaces.

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Area of Science:

Microscopy and Image Analysis
Cell Biology
Biophysics

Background:

High-dimensional fluorescence microscopy generates complex datasets.
Visualizing and quantifying data from spherical or uneven cellular surfaces is challenging.

Purpose of the Study:

To introduce Map3-2D, a novel software for projecting five-dimensional (5D) microscopy data onto 2D maps.
To enable accurate visualization and quantitative analysis of complex sample surfaces.

Main Methods:

Development of Map3-2D software for data projection.
Application of Map3-2D to fluorescence microscopy image stacks.
Utilizing the software for analysis of mammalian cells, yeast cells, and giant unilamellar vesicles.

Main Results:

Map3-2D successfully projects 5D image data onto structurally connected 2D maps.
The software facilitates visualization and quantitative analysis of both fixed and dynamic samples.
Demonstrated applicability on diverse biological samples including cells and vesicles.

Conclusions:

Map3-2D offers a powerful solution for visualizing and analyzing high-dimensional microscopy data.
The software enhances the study of surface structures and dynamics in biological systems.
Map3-2D is freely available, promoting wider use in scientific research.