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Updated: Apr 6, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
Come together, right now…
1Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada. hermie@sickkids.ca.
Abstract:
Editing the genome using approaches like TALEN and siRNA are already well tested. The new kid on the block is CRISPR-Cas9. CRISPR-Cas9 is rapidly evolving with impressive refinements for specificity, eliminating off-target effects, and versatility. One can adjust constructs and conditions to produce opposite effects on the genome and for a specific purpose. The nuances of the system, the means to significantly reduce off-targeting, and numerous applications are now emerging rapidly. This B&B commentary looks forward into how the CRISPR-Cas9 tool might serve the CCN field.
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