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Published on: October 10, 2014
Rapid and environmentally-friendly extraction of carotenoids from Blakeslea trispora
Hong-Bo Wang1, Li-Wei Zhang2, Jun Luo2
1School of Life Sciences, Hubei Province Engineering Research Center for Legume Plants, Jianghan University, Wuhan, 430056, China.
Objective:
A rapid and environmentally-friendly method of carotenoid extraction from Blakeslea trispora was performed using low-pressure steam explosion for cell disruption and ethyl lactate for the extraction.
Results:
Response surface methodology determined the optimal extraction conditions to obtain the maximum carotenoid yield. These optimal conditions consisted of low-pressure steam explosion at 0.2 MPa for 4.4 min, followed by extraction with ethyl lactate at a ratio of 1:25 at 39 °C for 45 min. Optimal technology was used, and the total carotenoid yield reached 95.6 %.
Conclusion:
In contrast to previously methods, the time-consuming drying process was eliminated and the extraction time was significantly decreased. Ethyl lactate is an environmentally-friendly solvent with low toxicity in this method.
