A simplified method for culture of human fetal heart tissue

Human fetal heart tissue obtained consequent to suction termination of pregnancy between 6 and 12 weeks of gestation were cultured as explants and maintained in a viable state, with spontaneous contractions up to 75 days. Ultrastructural morphology of the explant revealed that the cells remained healthy up to 21 days in culture. The model can therefore be used for experimental studies during the first 3 weeks in culture.