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Related Concept Videos

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Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
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Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
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Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
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Distinct SagA from Hospital-Associated Clade A1 Enterococcus faecium Strains Contributes to Biofilm Formation.

F L Paganelli1, M de Been1, J C Braat1

  • 1Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Applied and Environmental Microbiology
|July 26, 2015
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Extracellular DNA (eDNA) is crucial for Enterococcus faecium biofilm formation, while secreted antigen A (SagA) plays a variable role depending on its repeat profile, impacting treatment strategies for these nosocomial infections.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Enterococcus faecium is a significant cause of hospital-acquired infections, often forming biofilms.
  • Understanding biofilm development is key to combating these infections.
  • Extracellular DNA (eDNA) and proteins are known biofilm matrix components in bacteria.

Purpose of the Study:

  • To investigate biofilm formation in E. faecium strains from clades A1 and B.
  • To determine the roles of eDNA and secreted proteins in E. faecium biofilm development.
  • To explore the contribution of Secreted Antigen A (SagA) to biofilm structure and susceptibility to degradation.

Main Methods:

  • Assessed biofilm formation in 83 E. faecium strains.
  • Utilized DNase I and proteinase K to degrade eDNA and proteins in biofilms.
  • Analyzed the abundance and localization of SagA.
  • Sequenced sagA gene to examine repeat region variations.

Main Results:

  • eDNA was essential for biofilm formation in most E. faecium strains studied.
  • Proteolysis primarily affected biofilms of E. faecium clade A1 strains.
  • Secreted Antigen A (SagA) was abundant in both clades, but its localization and contribution varied.
  • Variations in the sagA repeat region correlated with biofilm susceptibility to proteinase K.

Conclusions:

  • Extracellular DNA is a critical component of Enterococcus faecium biofilms.
  • The role of secreted proteins, particularly SagA, in biofilm structure is influenced by genetic variations.
  • These findings highlight potential targets for novel anti-biofilm therapies against E. faecium infections.