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Published on: July 14, 2023
Simultaneous detection of 33 Streptococcus suis serotypes using the luminex xTAG® assay™
Xuemei Bai1, Zhijie Liu1, Shaobo Ji1
1State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
Abstract:
We report the development and evaluation of a serotype-specific xTAG luminex assay (SSA) that allows detection of the 33 serotypes of Streptococcus suis (S. suis). This assay is based on wzy gene targets directly involved in the cps biosynthesis and can be completed 40 min post-PCR amplification. The assay correctly and specifically identified the serotype of all 209 isolates tested, in comparison with two serotyping multiplex PCR methods previously developed. The sensitivity was higher than that of the previously described methods. The SSA system described here provides an easy-to-use, high-throughput system for rapid detection of S. suis serotypes.
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