A new organic matter fractionation methodology for organic wastes: Bioaccessibility and complexity characterization...

Organic matter characterization is the starting point to describe organic waste treatments mechanisms and to propose their modeling. Two relevant characterization methodologies were frequently used in the literature based on chemical extractions and fluorescence spectroscopy. However, they could not be generalized to all the type of wastes because of the different molecules targeted. Consequently, a new fractionation methodology was proposed to unify the characterization of a wide range of organic wastes. This new method was built by merging the two previously mentioned protocols to simulate bioaccessibility combined with 3D fluorescence spectroscopy to highlight the complexity of the extracted organic fractions. Sixty samples including representative samples used to validate the method were characterized according to their bioaccessibility and their complexity. Thanks to a principal component analysis, organic wastes were classified according to their nature, their complexity and accessibility. The applicability of this method in statistical or dynamic models is very promising.