Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systemic juvenile idiopathic arthritis: are there any predictors of disease course?

Rheumatology (Oxford, England)·2026
Same author

Students' engagement with AI-supported learning and its association with academic interest and career intentions in business analytics education.

PloS one·2026
Same author

Genetic and genomic analyses of tree architectural traits in Hevea brasiliensis revealed genes underlying QTLs linked to key developmental processes.

PloS one·2026
Same author

Optimization of somatic embryogenesis protocol for Hevea brasiliensis clones RRIM 600 and REYAN 88-13.

Journal, genetic engineering & biotechnology·2026
Same author

Role of anakinra in the management of children with severe dengue.

Archives of disease in childhood·2025
Same author

Micrografting Technique of <i>Hevea brasiliensis</i> in Vitro Plantlets.

Bio-protocol·2025

Related Experiment Video

Updated: Apr 6, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

13.4K

Development of a new pCAMBIA binary vector using Gateway® technology.

Julie Leclercq1, Toth Szabolcs1, Florence Martin1

  • 1CIRAD, UMR AGAP, F-34398 Montpellier, France.

Plasmid
|July 27, 2015
PubMed
Summary

Researchers developed a new Gateway®-compatible pCAMBIA vector, pCamway35S, for plant transformation. This novel vector demonstrates efficient gene delivery in Allium cepa and Hevea, matching existing standards.

Keywords:
Genetic transformationGreen fluorescent protein

More Related Videos

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

17.4K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.3K

Related Experiment Videos

Last Updated: Apr 6, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

13.4K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

17.4K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

15.3K

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetic Engineering

Background:

  • pCAMBIA vectors are widely used in plant science due to their stability and versatility.
  • The Gateway® cloning system offers efficient, site-specific recombination, simplifying cloning procedures.
  • Integrating Gateway® into pCAMBIA vectors can enhance plant transformation workflows.

Purpose of the Study:

  • To develop a novel Gateway®-compatible binary vector based on pCAMBIA2300.
  • To create the pCamway35S destination vector incorporating the Gateway® cassette under the CaMV35S promoter.
  • To evaluate the efficiency of the pCamway35S vector in plant transformation systems.

Main Methods:

  • Conversion of the pCambia2300 binary vector to include the Gateway® cassette.
  • Construction of the pCamway35S destination vector driven by the CaMV35S promoter.
  • Assaying transformation efficiency using the uidA reporter gene in Allium cepa and Hevea embryogenic calli via particle bombardment and Agrobacterium tumefaciens.

Main Results:

  • Successful generation of the pCamway35S destination vector.
  • Demonstrated successful transient and stable transformation in Allium cepa and Hevea.
  • Statistical analysis confirmed pCamway35S::uidA efficiency comparable to the established pCambia2301 vector.

Conclusions:

  • The pCamway35S vector is a functional and efficient tool for plant transformation.
  • This new vector integrates the benefits of the Gateway® system with pCAMBIA's utility.
  • pCamway35S offers a valuable alternative for researchers seeking streamlined plant genetic manipulation.