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Related Experiment Videos

A potexvirus isolated from Silene pratensis.

D A Wakarchuk1, R I Hamilton

  • 1Agriculture Canada Research Station, Vancouver, B.C., Canada.

Intervirology
|January 1, 1989
PubMed
Summary
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Silene virus X (SVX), a potexvirus infecting Silene pratensis, was characterized. This plant virus shows no homology with other potexviruses but is serologically related to narcissus mosaic virus.

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Phytopathology

Background:

  • Silene virus X (SVX) is a newly identified potexvirus.
  • This virus was isolated from infected weed species, Silene pratensis.

Purpose of the Study:

  • Characterize the physical and molecular properties of SVX.
  • Determine the phylogenetic relationship of SVX to other potexviruses.

Main Methods:

  • Isolation and purification of SVX particles.
  • RNA extraction and characterization (ssRNA, dsRNA).
  • In vitro translation assays.
  • Serological assays and cDNA hybridization.

Main Results:

  • Infectious SVX preparations contained nucleoprotein rods (546 nm length) with 6.9-kb polyadenylated ssRNA and 29-kd capsid protein.

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  • dsRNAs of 17.1, 7.0, 5.7, and 4.6 kbp were identified in infected plants.
  • SVX showed no nucleotide homology with seven other potexviruses.
  • SVX is serologically related to narcissus mosaic virus.

    • Conclusions:

    • SVX represents a distinct potexvirus species.
    • SVX is phylogenetically distant from other characterized potexviruses.
    • Further research is needed to understand SVX's impact on host plants.