Molecular cloning and physical mapping of the DNA of Miscanthus streak geminivirus

Molecular cloning and physical mapping of miscanthus streak virus (MiSV) circular double-stranded (ds) DNA was carried out. The circular dsDNA was isolated from leaves of Miscanthus saccariflorus (Gramineae) infected with MiSV. In addition to the monomeric circular dsDNA form in MiSV-infected leaves, there were minor circular dsDNA populations of twice (dimer) and approximately half genome length. The size of monomeric circular dsDNA was about 2.67 kilobase pairs. The evidence presented suggests that the genome of MiSV consists of one component.