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Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

What is Genetic Engineering?

What is Genetic Engineering?

Transgenic Organisms

Transgenic Organisms

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Apr 6, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

Genetically modified proteins: functional improvement and chimeragenesis.

Larissa Balabanova^1,2, Vasily Golotin^1,2, Anna Podvolotskaya²

¹a G.B. Elyakov Pacific Institute of Bioorganic Chemistry; Far Eastern Branch; Russian Academy of Science ; Vladivostok , Russia.

|July 28, 2015

Summary

Site-specific mutagenesis and chimeragenesis offer new protein engineering avenues. These advanced genetic modification techniques create novel biomolecules for diagnostics, therapeutics, and biocatalysis.

Keywords:

activity antibodies enzymes epitopes hybrid proteins lectins molecular structure site-specific mutagenesis specificity

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Area of Science:

Protein engineering
Biotechnology
Molecular biology

Background:

Traditional protein engineering methods are reaching their limits.
Advancements in structural biology, particularly X-ray crystallography, provide insights into protein structures and mutations.
There is a need for novel protein engineering strategies to overcome existing limitations.

Purpose of the Study:

To review the emerging roles of site-specific mutagenesis and chimeragenesis in protein functional improvement.
To highlight the potential of these techniques in creating novel proteins with enhanced properties.
To showcase applications in diagnostics, biotherapeutics, and biocatalysis.

Main Methods:

Focus on site-specific mutagenesis and chimeragenesis techniques.
Leveraging structure and sequence optimization algorithms.
Utilizing data from X-ray crystallography studies of proteins and their mutants.
In vivo and in vitro genetic improvement strategies.

Main Results:

Demonstration of novel protein creation through advanced genetic modifications.
Identification of potential applications for engineered proteins in diagnostics, biotherapeutics, and biocatalysis.
Examples of in vivo and in vitro functional improvement of binding proteins and enzymes.

Conclusions:

Site-specific mutagenesis and chimeragenesis represent a significant advancement in protein engineering.
These methods expand the possibilities for designing proteins with desired functions.
The engineered proteins hold promise for various biotechnological and biomedical applications.