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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Apr 6, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.

Andrew B Lane¹, Magdalena Strzelecka¹, Andreas Ettinger²

¹Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA.

Developmental Cell

|July 28, 2015

Summary

This summary is machine-generated.

Researchers developed an affordable CRISPR RNA guide generation method for whole-genome screening. This new technique enables efficient labeling of repetitive or single chromosomal loci in Xenopus egg extracts and targets bacterial genomes.

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Published on: December 6, 2017

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

CRISPR-based technologies offer powerful genome alteration capabilities.
A need exists for cost-effective methods to generate numerous RNA guides for large-scale screening and labeling applications.

Purpose of the Study:

To develop an inexpensive method for generating large libraries of CRISPR RNA guides.
To demonstrate the utility of these libraries for genomic labeling and screening.

Main Methods:

A novel library construction method utilizing any DNA source was employed.
Guide libraries were generated and tested in Xenopus egg extracts.
The targeting efficiency of complex libraries against the E. coli genome was assessed.

Main Results:

The method successfully generated guide libraries capable of labeling repetitive and single chromosomal loci.
A complex guide library demonstrated high-frequency targeting of the E. coli genome.
The approach proved effective in Xenopus egg extracts, indicating broad applicability.

Conclusions:

The developed CRISPR RNA guide generation method is inexpensive and scalable.
This technique facilitates whole-genome screening and chromosomal labeling.
The findings present a significant advancement for CRISPR-based genomic applications.