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[The plasmids of Pseudomonas syringae]
Izvestiia Akademii Nauk SSSR. Seriia Biologicheskaia
|November 1, 1989
Summary
Plasmids are common in Pseudomonas syringae plant pathogens from various regions, found in both virulent and avirulent strains. However, specific P-2 plasmids were absent in the studied Pseudomonas syringae pathovars.
Area of Science:
- Microbiology
- Plant Pathology
- Bacteriology
Background:
- Pseudomonas syringae is a significant plant pathogen with diverse pathovars.
- Plasmids are known to influence bacterial virulence and adaptation.
- Understanding plasmid occurrence in P. syringae is crucial for disease management.
Purpose of the Study:
- To investigate the presence and diversity of plasmids in P. syringae strains.
- To determine if plasmid carriage correlates with virulence in P. syringae.
- To identify plasmid incompatibility groups within P. syringae populations.
Main Methods:
- Isolation and characterization of P. syringae strains from various geographical locations.
- Plasmid DNA extraction and analysis.
- Determination of plasmid molecular weights and incompatibility groups.
Main Results:
- Plasmids were detected in P. syringae strains from twelve out of seventeen pathovars studied.
- One to four distinct plasmids, ranging from 20 to 90 Md, were identified.
- Plasmids were present in both virulent and avirulent strains of P. syringae.
- Plasmids belonging to incompatibility group P-2 were not found in any of the examined strains.
Conclusions:
- Plasmid carriage is a common feature among various P. syringae pathovars.
- The presence of plasmids does not appear to be solely linked to virulence in P. syringae.
- The absence of P-2 plasmids suggests specific evolutionary or ecological constraints.