Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM)

Area of Science:

• Biochemistry
• Materials Science
• Chemical Engineering

Background:

• Eggshell membrane is a rich source of collagen.
• Efficient extraction of pepsin-soluble collagen (PSC) is crucial for its utilization.
• Optimization of extraction parameters is needed to maximize PSC yield.

Purpose of the Study:

• To investigate the effect of extraction variables on PSC yield from eggshell membrane.
• To determine the optimal conditions for maximizing PSC extraction using response surface methodology.

Main Methods:

• Response surface methodology (RSM) with a central composite design (CCD).
• Investigated NaOH concentration, alkali treatment time, enzyme concentration, and hydrolysis time.
• Fitted experimental data to a second-order polynomial equation using multiple regression analysis.

Main Results:

• Identified optimal extraction conditions: 0.76 mol/l NaOH, 18 h alkali treatment, 50 U/mg enzyme, and 43.42 h hydrolysis.
• Achieved an experimental PSC extraction yield of 30.049% under optimal conditions.
• Experimental yield closely matched the predicted value (30.054%).

Conclusions:

• Response surface methodology effectively optimized PSC extraction from eggshell membrane.
• The established optimal conditions provide a reliable method for maximizing collagen yield.
• This research contributes to the efficient recovery and potential application of eggshell membrane collagen.