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Updated: Apr 6, 2026
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Development and validation of a modified QuEChERS protocol coupled to LC-MS/MS for simultaneous determination of
Amr H Shendy1, Medhat A Al-Ghobashy2, Sohair A Gad Alla1
1Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food, Agricultural Research Center, Ministry of Agriculture and Land Reclamation, Giza, Egypt.
Abstract:
LC-MS/MS assay was developed and validated according to EU guidelines for determination of nitrofuran metabolites and nitroimidazole residues in honey. Crude samples were acid-treated to liberate matrix-bound residues and a modified QuEChERS protocol was employed. Nitrofurantoin, furazolidone, furaltadone and nitrofurazone were determined via analysis of their metabolites AHD, AOZ, AMOZ and SEM, respectively while nitroimidazole residues; ronidazole (RNZ) and dimetridazole (DMZ) were determined directly. For all analytes, neat standard calibration curves, after correction for matrix effect were successfully employed. Decision limit (CCα) and detection capability (CCβ) were below the MRPL for nitrofurans (1.00 μg kg(-1)) and the recommended concentration for nitroimidazole (3.00 μg kg(-1)), respectively. The CCα, CCβ, percentage recovery and CV% ranges were 0.12-0.74 μg kg(-1), 0.21-1.27 μg kg(-1), 90.96-104.80% and 2.65-12.58%, respectively. This work is part of the national initiative for establishing a national monitoring program for drug residues in Egyptian honey.
