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This study optimized a high-performance liquid chromatography method for analyzing chlorogenic acids in yerba mate. The best results were achieved using specific methanol concentrations and gradient times for accurate quantification.

Keywords:
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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Natural Product Analysis

Background:

  • Yerba mate (Ilex paraguariensis) is rich in bioactive compounds, particularly chlorogenic acids.
  • Accurate quantification of these compounds is essential for understanding their health benefits.
  • Existing analytical methods may require optimization for complex plant matrices.

Purpose of the Study:

  • To develop and optimize a reverse-phase high-performance liquid chromatographic (RP-HPLC) method.
  • To achieve simultaneous separation and accurate quantification of multiple chlorogenic acids and rutin.
  • To optimize the method for aqueous extracts of yerba mate.

Main Methods:

  • Central composite design (CCD) was employed for experimental design.
  • Optimization of mobile phase composition (methanol concentration) and gradient elution time.
  • Desirability function methodology was used for simultaneous multi-response optimization.

Main Results:

  • Optimal conditions involved lower initial and final methanol concentrations and longer gradient times.
  • The optimized method achieved improved peak resolution and symmetry.
  • The method was successfully applied to analyze various yerba mate preparations (chimarrão, tererê, mate tea).

Conclusions:

  • The developed RP-HPLC method is efficient for simultaneous analysis of key compounds in yerba mate.
  • Yerba mate extracts are confirmed as excellent sources of chlorogenic acids.
  • The optimized method provides a reliable tool for quality control and research on yerba mate products.