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Updated: Apr 6, 2026

A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Technologies for Single-Cell Isolation.

Andre Gross^1,2, Jonas Schoendube^3,4, Stefan Zimmermann⁵

¹Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, Freiburg 79110, Germany. andre.gross@imtek.uni-freiburg.de.

International Journal of Molecular Sciences

|July 28, 2015

Summary

This review covers technologies for single-cell isolation, crucial for cell line development and cancer research. Fluorescence activated cell sorting (FACS) and other methods are detailed, with future trends analyzed.

Keywords:

flow cytometry laser microdissection limiting dilution microfluidics single-cell analysis single-cell handling single-cell separation single-cell technologies

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Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

Single-cell handling is vital for cell line development, single-cell analysis, cancer research, and diagnostics.
Various technologies exist for isolating single cells, with ongoing development for improved methods.

Purpose of the Study:

To provide a comprehensive overview of current and emerging single-cell isolation technologies.
To present findings from a market survey on the most relevant single-cell isolation technologies.
To identify future trends and potentially relevant emerging technologies through patent analysis.

Main Methods:

Review of existing literature and technologies for single-cell isolation.
Conducting an online market survey to determine the usage frequency of different technologies.
Performing a worldwide patent search to identify novel and emerging technologies.

Main Results:

Fluorescence activated cell sorting (FACS)/Flow cytometry (33%), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip (12%) are the most frequently used technologies.
Key performance factors of prominent technologies are discussed.
A growing interest in single-cell isolation tools is anticipated in the coming years.

Conclusions:

The review details prominent single-cell isolation technologies and their performance metrics.
Market survey data highlights current technology preferences and usage.
Patent analysis suggests a dynamic field with emerging innovations for future single-cell analysis applications.