Efficient and quantitative high-throughput tRNA sequencing
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Updated: Apr 6, 2026

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
Published on: February 23, 2024
Guanqun Zheng1, Yidan Qin2, Wesley C Clark1
1Department of Biochemistry & Molecular Biology, the University of Chicago, Chicago, IL 60637, USA.
Abstract:
Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.
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