Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A primer on regulation of laboratory-developed testing procedures: A points to consider statement of the American College of Medical Genetics and Genomics (ACMG).

Genetics in medicine : official journal of the American College of Medical Genetics·2025
Same author

Response to Spurdle et al.

Genetics in medicine : official journal of the American College of Medical Genetics·2023
Same author

Genomic and clinical characterization of early T-cell precursor lymphoblastic lymphoma.

Blood advances·2021
Same author

Correction: Technical standards for the interpretation and reporting of constitutional copy-number variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics (ACMG) and the Clinical Genome Resource (ClinGen).

Genetics in medicine : official journal of the American College of Medical Genetics·2021
Same author

Genomic landscape of cutaneous follicular lymphomas reveals 2 subgroups with clinically predictive molecular features.

Blood advances·2021
Same author

Seizures and Cardiomyopathy in a Patient with Pallister-Killian Syndrome due to Hexasomy 12p Mosaicism.

Molecular syndromology·2020

Related Experiment Video

Updated: Apr 6, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.7K

Streamlining the OncoScan® Array Procedure for Use in a Clinical Laboratory.

Christian N Paxton1, Leslie R Rowe1, Sarah T South1

  • 1ARUP Institute for Clinical and Experimental Pathology®, Salt Lake City, UT.

Journal of the Association of Genetic Technologists
|July 28, 2015
PubMed
Summary

This study optimized the OncoScan® assay for clinical labs. Modifications to the protocol maintained assay performance for detecting copy number changes (CNCs) and loss of heterozygosity (LOH).

More Related Videos

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

9.4K
Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
09:37

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

3.1K

Related Experiment Videos

Last Updated: Apr 6, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
09:16

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

20.7K
In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

9.4K
Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
09:37

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

3.1K

Area of Science:

  • Molecular Diagnostics
  • Genomic Analysis
  • Clinical Laboratory Science

Background:

  • Microarray analysis is crucial for detecting copy number changes (CNCs) and loss of heterozygosity (LOH) in clinical settings.
  • The OncoScan® array is a tool for identifying CNCs and LOH in formalin-fixed paraffin-embedded oncology samples.

Purpose of the Study:

  • To identify modifications to the OncoScan® procedure for improved efficiency and user-friendliness in clinical laboratories.
  • To evaluate the performance of a modified OncoScan® protocol compared to the manufacturer's standard protocol.

Main Methods:

  • Eighteen oncology samples were reprocessed using a modified OncoScan® protocol.
  • Key modifications included eliminating a "chill and spin" step and adjusting overnight hybridization temperature for simultaneous OncoScan® and CytoScan® array hybridization.

Main Results:

  • The modified protocol yielded comparable quality control metrics and diagnostic calls to the manufacturer-recommended protocol.
  • Paired sample analysis demonstrated equivalent performance between the standard and modified OncoScan® protocols.

Conclusions:

  • The optimized OncoScan® protocol is a viable alternative for clinical laboratories.
  • Modifications enhance efficiency and technician-friendliness without compromising diagnostic accuracy for CNCs and LOH detection.