Related Experiment Video
Updated: Apr 6, 2026
06:10
Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye
Published on: March 30, 2020
8.5K
Injectable PLGA microspheres encapsulating WKYMVM peptide for neovascularization.
Young Hwan Choi1, Soon Chul Heo2, Yang Woo Kwon2
1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 152-742, Republic of Korea.
Acta Biomaterialia
|July 29, 2015
Summary
WKYMVm peptide enhances blood vessel growth by activating formyl peptide receptor-2 (FPR-2). Sustained release from microspheres effectively restored blood flow in ischemic limbs, promoting capillary growth.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Vascular Biology
Background:
- Formyl peptide receptor-2 (FPR-2) is crucial for inflammation and angiogenesis.
- WKYMVm is a novel synthetic peptide agonist for FPR-2.
- Endothelial cells, including outgrowth endothelial cells (OECs), express FPR-2.
Purpose of the Study:
- To investigate the angiogenic potential of WKYMVm peptide.
- To evaluate the efficacy of WKYMVm peptide delivered via sustained-release microspheres for treating ischemic injury.
Main Methods:
- Assessed OEC migration, proliferation, and tube formation in response to WKYMVm.
- Utilized in vitro tube formation and aortic ring assays to study angiogenesis.
- Fabricated poly(lactide-co-glycolide) (PLGA) microspheres for sustained WKYMVm peptide release.
- Evaluated the therapeutic effect in a hind limb ischemia mouse model.
Main Results:
- WKYMVm peptide significantly stimulated OEC migration, proliferation, and tube formation.
- In vitro assays confirmed WKYMVm's pro-angiogenic activity.
- Injectable PLGA microspheres demonstrated sustained release of WKYMVm.
- A single injection of WKYMVm-loaded microspheres effectively restored blood flow and promoted capillary growth in ischemic hind limbs, comparable to multiple peptide injections.
Conclusions:
- WKYMVm peptide is a potent stimulator of angiogenesis.
- Sustained delivery of WKYMVm via PLGA microspheres enhances its therapeutic efficacy for ischemic conditions.
- This approach offers a promising strategy for regenerative medicine applications in ischemic diseases.
