In-vitro development of zygotes from superovulated prepubertal and mature gilts

Ten prepubertal and 8 mature gilts were superovulated with PMSG and hCG, and inseminated with fresh boar semen. Zygotes were surgically recovered from oviducts 54-60 h after hCG. One and 2-cell zygotes were randomly allotted to Medium PL (modified BMOC-3 supplemented with 0.1 mM-EDTA and 1.5% BSA) or Medium G (Medium PL without pyruvate or lactate). Eggs were washed twice in medium, and placed in microdrops of medium overlaid with silicon oil for culture in an humidified 5% CO2, 5% O2, 90% N2 environment, then observed daily for 6 days. Development of eggs was dependent (P less than 0.001) on the interactive effects of age of gilt (prepubertal versus mature) and medium type (PL versus G) used in culture. A greater proportion of eggs cultured in Medium G developed further than did eggs in Medium PL (P less than 0.001). Additionally, a greater proportion of eggs from mature gilts developed further than did eggs from prepubertal gilts (P less than 0.02). We suggest that these results provide evidence that zygotes resulting from superovulation regimens of prepubertal gilts do not possess the same capacity for in-vitro development as do zygotes from pubertal gilts.