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Data set for the mass spectrometry based exoproteome analysis of Aspergillus flavus isolates
Ramu Muthu Selvam1, Rathnavel Nithya1, Palraj Narmatha Devi1
1Proteomics Department, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India.
Abstract:
Aspergillus flavus is one of the predominant causative organisms of mycotic keratitis in tropical parts of the world. Extracellular proteins are the earliest proteins that come in contact with the host and have a role in the infection process. Exoproteins of A. flavus isolated from infected cornea, sputum and a saprophyte were pooled and identified using high resolution mass spectrometry in order to get the total exoproteome from cultures isolated from different sources. A total of 637 proteins was identified from the pooled A. flavus exoproteome. Analysis based on GO annotations of the 637 identified proteins revealed that hydrolases form the predominant class of proteins in the exoproteome. Interestingly, a greater proportion of the exoproteins seem to be secreted through the non-classical pathways. This data represent the first in-depth analysis of the representative A. flavus exoproteome of a large set of isolates from distinct sources. This data have been deposited to the ProteomeXchange with identifier PXD001296.
Insights
This study identified 637 proteins in the exoproteome of Aspergillus flavus, a common cause of fungal eye infections. Hydrolases were the most abundant proteins, with many secreted via non-classical pathways.
Area of Science:
- Microbiology
- Proteomics
- Mycology
Background:
- Aspergillus flavus is a major cause of mycotic keratitis, particularly in tropical regions.
- Extracellular proteins (exoproteins) are crucial for initiating host-pathogen interactions during infection.
Purpose of the Study:
- To comprehensively identify and analyze the exoproteome of Aspergillus flavus from diverse sources.
- To understand the protein composition and secretion pathways of A. flavus exoproteins.
Main Methods:
- Pooled exoproteins from A. flavus isolates (cornea, sputum, saprophyte) were analyzed.
- High-resolution mass spectrometry was employed for protein identification.
- Gene Ontology (GO) annotations were used for functional analysis.
Main Results:
- A total of 637 proteins were identified in the pooled A. flavus exoproteome.
- Hydrolases constituted the predominant protein class.
- A significant proportion of exoproteins were found to be secreted via non-classical pathways.
Conclusions:
- This study provides the first in-depth analysis of a representative A. flavus exoproteome from multiple sources.
- The findings highlight the importance of hydrolases and non-classical secretion in A. flavus pathogenesis.
- The generated proteomic data is publicly available (ProteomeXchange identifier PXD001296).
