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Updated: Apr 6, 2026
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Quantification of proteome changes in bovine muscle from two-dimensional electrophoresis data
Daniel Franco1, Ariadna Mato2, Francisco J Salgado3
1Meat Technology Center of Galicia, r/Galicia 4, Parque Tecnolóxico de Galicia, San Cibrao das Viñas, Ourense 32900, Galicia, Spain.
Abstract:
Proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress were assessed on the basis of two-dimensional electrophoresis (2-DE) data. In this study, the bootstrap resampling statistical technique and a new measure of relative change of the volume of 2-DE protein spots are shown to be more efficient than commonly used statistics to reliably quantify changes in protein abundance in stress response. The data are supplied in this article and are related to "Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress" by Franco et al. [1].
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