Data for proteomic analysis of Human monocyte-derived macrophages

Area of Science:

• Immunology
• Proteomics
• Cell Biology

Background:

• Macrophages are crucial immune cells with diverse functions.
• Tissue macrophages are heterogeneous and challenging to study directly.
• In vitro models, like monocyte-derived macrophages (MDMs), are used to study macrophage biology.

Purpose of the Study:

• To investigate the proteomic differences between distinct subsets of in vitro-differentiated human monocyte-derived macrophages (MDMs).
• To provide a deeper understanding of macrophage heterogeneity using proteomic analysis.
• To support the research article 'Human monocyte-derived macrophages are heterogeneous: proteomic profile of different phenotypes' by Eligini et al.

Main Methods:

• Nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS(E)) was employed for proteomic analysis.
• Proteomic profiling was performed on two dominant subsets of spontaneously differentiated MDMs: round and spindle phenotypes.
• Data analysis focused on identifying and quantifying proteins expressed in each MDM subset.

Main Results:

• Significant differences in protein expression were observed between round and spindle MDM subsets.
• The proteomic data revealed distinct molecular signatures for each MDM phenotype.
• These differences in proteome contribute to the functional and transcriptional variations observed between MDM subsets.

Conclusions:

• Human monocyte-derived macrophages (MDMs) display significant proteomic heterogeneity.
• The distinct proteomic profiles of MDM subsets partially mimic the heterogeneity of tissue macrophages.
• This proteomic dataset enhances the comprehension of macrophage biology and heterogeneity in vitro.