Effects of wavelength error and spectral band width on measurement of alkaline phosphatase activity

We re-examined the effects of wavelength error and spectral band width on the measurement of alkaline phosphatase activity. The methods we studied is relatively insensitive to these two factors, a conclusion we base on both experimental results and theoretical analysis. These findings are in conflict with a recently published report [Lott et al., Clin. Chem. 24, 938 (1978)], and we suggest a possible explanation for this.