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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
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Updated: Apr 6, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
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Denitrification accelerates granular sludge formation in sequencing batch reactors.

E Suja1, Y V Nancharaiah1, T V Krishna Mohan2

  • 1Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam 603102, Tamil Nadu, India.

Bioresource Technology
|July 29, 2015
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Summary
This summary is machine-generated.

Denitrification accelerates aerobic granular sludge formation in wastewater treatment. This process involves nitric oxide signaling and calcium carbonate precipitation, enhancing microbial aggregation and granulation efficiency.

Keywords:
Aerobic granular sludgeDenitrificationNitric oxide signalingNitrogen removalSequencing batch reactor

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Area of Science:

  • Environmental microbiology
  • Wastewater treatment engineering
  • Biotechnology

Background:

  • Aerobic granular sludge (AGS) is crucial for efficient wastewater treatment.
  • Understanding factors accelerating AGS formation is vital for process optimization.
  • Denitrification's role in AGS development remains an area of active research.

Purpose of the Study:

  • To investigate the impact of denitrification on aerobic granular sludge formation in sequencing batch reactors (SBRs).
  • To elucidate the mechanisms by which denitrification influences sludge granulation.

Main Methods:

  • Comparison of AGS formation in SBRs with and without nitrate/nitrite feeding.
  • Sludge characterization including calcium content and mineral analysis.
  • Assessment of extracellular polymeric substances (EPS) production.
  • Utilizing sodium nitroprusside as a nitric oxide (NO) donor to study signaling pathways.

Main Results:

  • Faster AGS formation observed in reactors fed with nitrate or nitrite compared to controls.
  • Denitrification primarily occurred during the anoxic static fill phase.
  • Accumulation of calcium and calcium carbonate signatures identified in nitrate-fed SBRs.
  • NO donor enhanced microbial aggregation, EPS production, and AGS formation.

Conclusions:

  • Denitrification positively influences aerobic sludge granulation in SBRs.
  • Nitric oxide (NO) signaling and calcium carbonate (CaCO3) formation are key mechanisms facilitating granulation.
  • Nitrate and denitrification intermediates enhance microbial cell aggregation and accelerate AGS development.