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Related Experiment Video

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TiProtec preserves endothelial function in a rat model.

Gábor Veres1, Péter Hegedűs1, Enikő Barnucz2

  • 1Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany; Heart and Vascular Center, Semmelweis University, Budapest, Hungary.

The Journal of Surgical Research
|July 30, 2015
PubMed
Summary

TiProtec solution better preserves endothelium in bypass grafts compared to saline and Custodiol after cold storage and reperfusion. This novel solution enhances graft function and cell integrity, improving long-term outcomes in myocardial revascularization.

Keywords:
BypassEndotheliumGraftingIschemiaReperfusion

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Area of Science:

  • Vascular Surgery
  • Organ Preservation
  • Biomaterials Science

Background:

  • Coronary artery bypass grafting (CABG) outcomes are limited by graft failure.
  • Preserving graft endothelium during cold storage and warm reperfusion is critical.
  • Investigating novel preservation solutions is essential for improving CABG success rates.

Purpose of the Study:

  • To compare the efficacy of saline, Custodiol, and TiProtec solutions in preserving aortic graft endothelium.
  • To evaluate the impact of these solutions on endothelial function and cell integrity after cold ischemia and warm reperfusion.
  • To assess the long-term patency and functional recovery of grafts preserved with different solutions.

Main Methods:

  • Aortic transplantation in Lewis rats using grafts stored in saline, Custodiol, or TiProtec for 2 hours.
  • Assessment of endothelium-dependent and -independent vasorelaxation via organ bath studies.
  • Analysis of DNA strand breaks, mRNA expression (Bax, BCL2, eNOS, VEGF2, caspase-3), and CD-31/alpha-SMA expression via molecular and immunohistochemical techniques.

Main Results:

  • Saline and Custodiol significantly impaired endothelial function and graft integrity post-transplantation.
  • TiProtec demonstrated superior preservation of endothelial function and CD-31 expression compared to saline and Custodiol.
  • While all groups showed partial recovery at 1 week, TiProtec-preserved grafts exhibited significantly better endothelial function and integrity.

Conclusions:

  • TiProtec solution is more effective than saline and Custodiol in preserving endothelial and smooth muscle cells in bypass grafts.
  • This preservation is evident after both cold storage and warm reperfusion injury.
  • TiProtec shows promise for improving the long-term success of myocardial revascularization procedures.