Related Experiment Video
Updated: Apr 6, 2026
10:28
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
10.6K
A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Leticia Mara Lima Angelini1, Amanda Ribeiro Martins da Silva1, Lucas de Freitas Coli Rocco1
1Instituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
Abstract:
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Related Concept Videos
01:08Nitriles to Carboxylic Acids: Hydrolysis
5.5K
Nitriles undergo acid-catalyzed hydrolysis or base-catalyzed hydrolysis to form a carboxylic acid. These reactions proceed via an amide intermediate.
5.5K
01:12Preparation of Nitriles
2.8K
One of the common methods to prepare nitriles is the dehydration of amides. This method requires strong dehydrating agents like phosphorous pentoxide or boiling acetic anhydride for converting amides to nitriles. Another reagent namely, thionyl chloride also accomplishes the dehydration of amides, where amide acts as a nucleophile. The first step of the mechanism involves the nucleophilic attack by the amide on the thionyl chloride to form an intermediate. In the next step, the electron pairs...
2.8K