Related Experiment Video

Updated: Apr 6, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

Evaluating the microRNA-target gene regulatory network in renal cell carcinomas, identification for potential

Jun Li1, Jian-Hua Huang2, Qing-Hua Qu1

  • 1Department of Urology, Pudong New Area People's Hospital Shanghai, China.

International Journal of Clinical and Experimental Medicine
|July 30, 2015
PubMed

Insights

This study identified novel genes linked to renal cell carcinoma (RCC) by analyzing gene expression. Key genes and pathways were found, offering potential biomarkers and therapeutic targets for RCC.

Area of Science:

  • Oncology
  • Genomics
  • Bioinformatics

Background:

  • Aberrant microRNA (miRNA) expression is common in cancers.
  • Integrating miRNA and messenger RNA (mRNA) expression aids in understanding oncogenic pathways.

Purpose of the Study:

  • To identify novel genes associated with renal cell carcinoma (RCC).
  • To analyze gene functions and critical signaling pathways in RCC using DNA microarray data.

Main Methods:

  • Downloaded gene expression profile GSE6344 from Gene Expression Omnibus database (10 RCC samples, 10 controls).
  • Identified differentially expressed genes (DEGs) between RCC and control samples.
  • Performed Gene Ontology (GO) enrichment analysis, constructed a protein-protein interaction (PPI) network, and conducted pathway enrichment analysis.

Main Results:

  • Identified 521 downregulated and 473 upregulated DEGs in RCC samples.
  • Discovered 15 enhanced and 17 suppressed functions.
  • Identified high-degree PPI nodes (e.g., RHCG, RALYL, SLC4A1, UMOD, CA9).
  • Found DEGs significantly enriched in the cytokine and cytokine receptor pathway.

Conclusions:

  • The identified hub genes in RCC samples may serve as biomarkers.
  • These findings provide a foundation for developing combination therapy approaches for RCC.
Keywords:
Microarray profilingcritical pathwaysoncogenespotential biomarkersrenal cell carcinoma

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

3.0K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

12.1K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K