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Updated: Apr 6, 2026
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Published on: February 16, 2017
Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues
Steven D Scoville1, Karen A Keller2, Stephanie Cheng2
11] Medical Scientist Training Program, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA [2] Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA [3] The Ohio State University Comprehensive Cancer Center, The James Cancer Hospital &Solove Research Center, Columbus, Ohio 43210, USA.
Abstract:
We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.
