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Related Experiment Videos

Neutrophil activation during cell separation procedures.

M H Jackson1, A M Millar, J Dawes

  • 1Department of Medicine, Royal Infirmary, Edinburgh, UK.

Nuclear Medicine Communications
|December 1, 1989
PubMed
Summary
This summary is machine-generated.

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Choosing the right blood processing agent is crucial for diagnostic imaging. Dextran 70 minimizes neutrophil activation during leucocyte isolation, unlike other sedimentation agents, ensuring accurate infection detection via scintigraphy.

Area of Science:

  • Biomedical diagnostics
  • Cellular immunology
  • Nuclear medicine

Background:

  • Leucocytes labeled with radioactive isotopes (e.g., 111In, 99Tcm) are vital for infection detection using scintigraphy.
  • Accurate diagnostic imaging relies on preserving leucocyte function during isolation procedures.
  • Neutrophil activation, indicated by elastase release, can be a marker of cellular stress during sample preparation.

Purpose of the Study:

  • To evaluate the impact of different leucocyte isolation methods on neutrophil activation.
  • To identify erythrocyte sedimentation agents and density gradient media that minimize neutrophil elastase release.
  • To determine the optimal agent for leucocyte isolation to preserve neutrophil integrity for diagnostic applications.

Main Methods:

Related Experiment Videos

  • Incubation of whole blood with erythrocyte sedimentation agents (dextran 70, hydroxyethyl starch, methylcellulose) and density gradient media (Percoll, Mono-Poly Resolving Medium).
  • Measurement of neutrophil elastase release using radioimmunoassay at 21°C and 37°C.
  • Statistical analysis of elastase release data to assess significance.

    • Main Results:

    • Dextran 70 did not induce elastase release at 21°C, while hydroxyethyl starch and methylcellulose did (p ≤ 0.01).
    • All three sedimentation agents induced significant elastase release at 37°C.
    • Percoll and Mono-Poly Resolving Medium did not cause neutrophil elastase release at either temperature.

    Conclusions:

    • Density gradient media (Percoll, Mono-Poly Resolving Medium) do not activate neutrophils during isolation.
    • Certain erythrocyte sedimentation agents, particularly hydroxyethyl starch and methylcellulose, induce significant neutrophil activation.
    • Dextran 70 is the preferred erythrocyte sedimentation agent for leucocyte isolation when minimizing neutrophil activation is critical for diagnostic scintigraphy.