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Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging
Wei Qiang Ong1, Y Rose Citron, Joerg Schnitzbauer
1Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA. bo.huang@ucsf.edu.
Chemical Communications (Cambridge, England)
|July 31, 2015
Abstract:
Photoactivatable fluorescent proteins (PA-FPs) are widely used in live single-molecule super-resolution imaging but emit substantially fewer photons than organic dyes do. Herein, we show that in heavy water (D2O) instead of H2O, common PA-FPs emit 26-54% more photons, effectively improving the localization precision in super-resolution imaging.
