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Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Altered expression of monocyte and dendritic cell membrane-associated antigens following coronary angiography
Insights
Coronary angiography alters monocyte and dendritic cell surface antigen expression, specifically CD14, CD16, and CD45. Blood collection before this procedure is crucial for accurate flow cytometry analysis.
Area of Science:
- Immunology
- Cardiology
- Cell Biology
Background:
- Coronary angiography can trigger a systemic inflammatory response.
- The procedure's impact on monocyte and dendritic cell characteristics is not fully understood.
Purpose of the Study:
- To investigate the effect of coronary angiography on monocyte and dendritic cell counts.
- To assess changes in membrane-associated antigen expression on these cells post-procedure.
Main Methods:
- Blood samples were collected from 20 stable angina patients before and after coronary angiography.
- Flow cytometry was used to enumerate cells and evaluate antigen expression.
- ELISA quantified plasma levels of soluble CD14 and interleukin-6.
Main Results:
- Monocyte and dendritic cell counts remained unchanged post-angiography.
- Significant decreases in CD14 expression on Mon1 and Mon2 subsets were observed.
- Increased CD16 expression on Mon2 and Mon3 subsets and decreased CD45 expression on monocytic and dendritic cells were noted.
- Plasma IL-6 levels decreased significantly, while sCD14 reduction was not significant.
Conclusions:
- Coronary angiography induces significant changes in the surface expression of CD14, CD16, and CD45 on monocytes and dendritic cells.
- These findings highlight the necessity of collecting blood samples prior to coronary angiography for reliable functional analysis of these immune cells.
Introduction:
Coronary angiography is able to induce a systemic inflammatory response. We hypothesised that this procedure may affect monocyte and dendritic cell count and membrane-associated antigen expression.
Methods:
Blood samples were obtained before and immediately after coronary angiography in twenty patients with stable angina pectoris. Cell enumeration and antigen expression levels were evaluated by flow cytometry. Plasma levels of soluble CD14 and interleukin-6 were quantified by ELISA.
Results:
The absolute and relative numbers of circulating monocytes (Mon1, Mon2 and Mon3 subsets) and dendritic cells (myeloid and plasmacytoid subsets) were not significantly different pre-versus post-angiography. Expression of CD14 on Mon1 and Mon2 decreased significantly by 12.01% (P = 0.002) and 13.01% (P=0.012), respectively. CD16 expression on Mon2 (+10.53%; P=0.017) and Mon3 (+12.58%; P<0.001) increased. CD45 expressed by monocytic and dendritic cells was lowered (-5.80% and P = 0.001, -11.49% and P < 0.001, respectively). The level of plasma IL-6 decreased significantly (P = 0.002). The reduction in sCD14 was not significant (P = 0.054).
Conclusion:
Coronary angiography leads to changes in surface expression of CD14, CD16 and CD45. These findings underline the importance of blood collection prior to the angiographic procedure when aiming to study the functional analysis of monocyte and dendritic cell numbers by flow cytometry.
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