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Updated: Apr 6, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Reactor performance and microbial characteristics of CANON process with step-wise increasing of C/N ratio
Xiaojing Zhang1, Dong Li2, Yuhai Liang2
1a Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration , School of Material and Chemical Engineering, Zhengzhou University of Light Industry , Zhengzhou 450001 , People's Republic of China.
Abstract:
In this study, the nitrogen removal performance and microbial characteristics of completely autotrophic nitrogen removal over nitrite (CANON) process was investigated with a step-wise increasing of C/N ratio (0.5, 1, 2 and 4) in a membrane bioreactor. The microbial distribution of aerobic ammonia-oxidizing bacteria (AOB) and anaerobic AOB (AAOB) was analysed by fluorescence in situ hybridization (FISH). Results showed that the denitrification ratio rose up correspondingly with the increase of influent C/N, and nitrogen removal rate (NRR) reached the maximum when C/N was 1 due to the harmonious work of denitrification and CANON. However, NRR decreased when influent C/N was more than 2. The threshold C/N ratio of CANON process was 2.2; so the sewage with a high C/N ratio should be pretreated by combining with pre-oxidation of organics or anaerobic-energy-producing process. FISH results showed decreasing numbers of both AOB and AAOB with the addition of organics.
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