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Two-photon microscopy of a Flt1 peptide-hyaluronate conjugate
Songeun Beack1, Jun-Sub Choi2, Jun Ho Lee3
1Department of Materials Science & Engineering, Pohang University of Science & Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Korea.
Aim:
Two-photon microscopy was performed to visualize ocular distribution of Flt1 peptide-hyaluronate (HA) conjugate micelles for eye drop treatment of corneal neovascularization.
Materials & Methods:
Flt1 peptide-HA conjugate micelles were topically administered to the eye for two-photon microscopy and antiangiogenic effect assessment after silver nitrate cauterization.
Results:
In vivo two-photon microscopy revealed that Flt1 peptide-HA conjugate micelles were absorbed and remained on the corneal epithelia with an increased residence time, facilitating the corneal delivery of carboxyfluorescein succinimidyl ester (CFSE) as a model drug. Furthermore, repeated eye drops of Flt1 peptide-HA conjugate micelles showed comparable therapeutic effect to the subconjunctival injection on the corneal neovascularization.
Discussion & Conclusion:
We confirmed the feasibility of Flt1 peptide-HA conjugate micelles for eye drop treatment of corneal neovascularization.
