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Related Experiment Video

Updated: Apr 6, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

Proteomic Approach to Identify Nuclear Proteins in Wheat Grain.

Emmanuelle Bancel^1,2, Titouan Bonnot^1,2, Marlène Davanture³

¹INRA, UMR1095 Genetics, Diversity and Ecophysiology of Cereals, 5 chemin de Beaulieu, F-63 039 Clermont-Ferrand, France.

Journal of Proteome Research

|August 1, 2015

Summary

Researchers analyzed the nuclear proteome in wheat grain during early development. They identified 528 unique proteins, many involved in DNA/RNA metabolism, revealing dynamic nuclear functions in developing wheat grains.

Keywords:

LC−MS/MS bread wheat (Triticum aestivum)cereal einkorn wheat (Triticum monococcum)grain development nuclear proteome

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Last Updated: Apr 6, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

mRNA Interactome Capture from Plant Protoplasts

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Area of Science:

Plant Biology
Proteomics
Molecular Biology

Background:

The wheat grain nucleus plays a crucial role in early development.
Understanding its proteome is key to deciphering gene regulation and cellular processes.

Purpose of the Study:

To comprehensively analyze the nuclear proteome of two wheat species (Triticum aestivum and T. monococcum) during early grain development.
To identify proteins involved in essential metabolic pathways and uncover novel proteins with unknown functions.

Main Methods:

Optimized nuclear purification and protein sample preparation techniques.
Utilized shotgun-based proteomics and MS/MS analysis for protein identification and quantification.

Main Results:

Identified 797 proteins, representing 528 unique proteins in wheat grain nuclei.
36% of identified proteins are involved in DNA and RNA metabolism.
Discovered 107 proteins with unknown or hypothetical functions.
Found 368 proteins common to both species and developmental stages, with some qualitative differences observed.

Conclusions:

The study provides a detailed map of the wheat grain nuclear proteome during early development.
Highlights the dynamic nature and functional complexity of the grain nucleus.
Identified key proteins involved in nucleic acid metabolism and numerous proteins requiring further functional characterization.