Reduced behavioral responses to intranigral muscimol following chronic ethanol

Increased biochemical measures of GABA activity are observed after acute administration of ethanol and decreased activity has sometimes been observed after chronic ethanol exposure. Since chronic alterations in neurotransmitter activity may result in changes in receptor function, it is possible that changes in GABA-receptive neurons may accompany chronic ethanol treatment. In the present study we examined the incidence of muscimol-induced motor behaviors in ethanol-naive and chronic ethanol-treated animals. Male Sprague-Dawley rats received bilateral cannula implants into substantia nigra pars reticulata for subsequent administration of muscimol or saline. After recovery from surgery, rats received chronic treatment in ethanol-vapor inhalation chambers for 15 days. Animals were then removed from the chambers and examined 10 hours after removal. Muscimol resulted in a general increase in motility in both control and ethanol-treated animals. Animals withdrawn from chronic ethanol exposure, however, exhibited significantly less muscimol-stimulated, repetitive 9 Hz movements. These results suggest that GABA receptive cells within the substantia nigra or its vicinity may be functionally less responsive to GABAergic stimulation after chronic ethanol administration.