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Reduction in oxidative stress biomarkers after adenotonsillectomy
Jae Hoon Cho1, Jeffrey D Suh2, Yong Won Kim1
1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Konkuk University, Seoul, South Korea.
Insights
Children with sleep disordered breathing have high levels of urinary 8-hydroxy-2-deoxyguanosine (8-OxodG). Adenotonsillectomy significantly reduced 8-OxodG and F(2)-isoprostane levels, indicating urine analysis can measure oxidative stress.
Area of Science:
- Pediatric Otolaryngology
- Biochemistry
- Clinical Diagnostics
Background:
- Sleep disordered breathing (SDB) and conditions like adenoid hypertrophy are linked to oxidative stress.
- Elevated serum oxidants are observed in various otolaryngic conditions.
Purpose of the Study:
- To quantify changes in urinary oxidative stress biomarkers in children following adenotonsillectomy.
- To investigate the correlation between SDB and specific urine markers of oxidative damage.
Main Methods:
- Collected urine samples from 22 children with SDB and 20 healthy controls.
- Measured urinary 8-hydroxy-2-deoxyguanosine (8-OxodG), F(2)-isoprostane, and malondialdehyde (MDA) using high-performance liquid chromatography.
- Repeated urine sample collection 3 weeks post-adenotonsillectomy for the SDB group.
Main Results:
- Preoperative urinary 8-OxodG levels were significantly higher in children with SDB compared to controls.
- Post-adenotonsillectomy, urinary 8-OxodG levels decreased to control levels.
- Urinary F(2)-isoprostane levels, initially similar, also decreased significantly after surgery.
Conclusions:
- Children with SDB exhibit elevated urinary 8-OxodG, a key marker of oxidative stress.
- Adenotonsillectomy effectively reduces oxidative stress markers (8-OxodG and F(2)-isoprostane) in children with SDB.
- Urinary biomarker analysis offers a promising, non-invasive method for assessing oxidative stress in pediatric populations.
Objectives:
A number of otolaryngic conditions such as chronic tonsillitis, adenoid hypertrophy, and obstructive sleep apnea are associated with oxidative stress and elevated levels of serum oxidants. The objective of this study is to measure changes in urine biomarkers of oxidative stress in children after adenotonsillectomy.
Methods:
Twenty-two children with sleep disordered breathing (SDB) with tonsil and adenoid hypertrophy were enrolled prior to adenotonsillectomy. Controls consisted of 20 healthy children. Urine samples were collected from all patients. Levels of three urinary biomarkers for oxidative status, 8-hydroxy-2-deoxyguanosine (8-OxodG), F(2)-isoprostane, and malondialdehyde (MDA) were measured using high performance liquid chromatography. For the study group, urine samples were repeated 3 weeks after surgery.
Results:
In the study group, preoperative urinary levels of 8-OxodG were higher than in controls (p=0.015). Levels decreased after surgery compared to preoperative levels (p=0.002), and reached control levels (p=0.167) at 3 weeks. Levels of urinary F(2)-isoprostane were similar in both groups (p=0.252), but decreased significantly after surgery (p=0.020).
Conclusions:
Children with SDB have elevated levels of urinary 8-OxodG, a marker of oxidative stress. Adenotonsillectomy results in decreased 8-OxodG and F(2)-isoprostane. These findings suggest that urine analysis may represent a valuable tool for the measurement of oxidative stress.
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