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Related Experiment Video

Updated: Apr 6, 2026

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

Transcriptome analysis highlights the conserved difference between embryonic and postnatal-derived alveolar

Sophie L Gibbings¹, Rajni Goyal¹, A Nicole Desch²

¹Department of Pediatrics.

|August 2, 2015

Summary

The lung environment largely shapes alveolar macrophage (AM) function and development. However, a few specific genes, like Marco, retain expression linked to their cellular origin, not their surroundings.

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Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

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Published on: April 20, 2018

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

Published on: June 25, 2016

Area of Science:

Immunology
Cell Biology
Respiratory Medicine

Background:

Alveolar macrophages (AMs) are crucial for lung defense and homeostasis.
AMs are primarily self-renewing, with limited monocyte replacement except in injury.
Understanding factors influencing AM development and function is vital for lung health.

Purpose of the Study:

To investigate the roles of cellular origin versus environmental factors in AM development and function.
To identify genes whose expression is dictated by origin or environment in AMs.

Main Methods:

Transcriptome analysis of coexisting embryonic and postnatal-derived AMs within the same mouse.
Functional analyses of AMs from different origins and environments.

Main Results:

AMs showed >98% transcriptome correlation and similar functions, indicating environment largely dictates programming.
A small subset of genes, notably Marco, exhibited origin-specific expression independent of the environment.
Marco gene expression differed between embryonic-derived and postnatal-derived macrophages.

Conclusions:

Under homeostatic conditions, the lung environment is the primary determinant of AM programming and function.
Cellular origin influences the expression of a limited number of genes in AMs, irrespective of the environment.