Related Experiment Video

Updated: Apr 6, 2026

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease
08:15

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

1.0K

Apoptotic Effect of Geniposide on Fibroblast-Like Synoviocytes in Rats with Adjuvant-Induced Arthritis via Inhibiting

Rong Li1, Li Cai2, Wen-Jian Tang1

  • 1School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, China.

Inflammation
|August 3, 2015
PubMed

Insights

Geniposide effectively induces apoptosis in fibroblast-like synoviocytes (FLS) from rats with rheumatoid arthritis. This compound regulates apoptosis-related gene expression and inhibits the ERK signaling pathway, offering a potential therapeutic strategy.

Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Rheumatoid arthritis (RA) involves fibroblast-like synoviocyte (FLS) proliferation.
  • Geniposide has demonstrated in vivo anti-arthritic effects.
  • Mechanisms of geniposide's action on RA FLS apoptosis remain unclear.

Purpose of the Study:

  • To investigate geniposide's ability to induce apoptosis in adjuvant-induced arthritis (AIA) rat FLS.
  • To elucidate the underlying molecular mechanisms of geniposide-induced apoptosis in AIA FLS.

Main Methods:

  • Adjuvant-induced arthritis (AIA) rat model established, FLS isolated via tissue explant.
  • MTT assay, Hoechst staining, and flow cytometry assessed apoptosis.
  • Real-time PCR and Western blot analyzed gene and protein expression (Bcl-2, Bax, caspase 3, ERK/p-ERK).

Main Results:

  • Geniposide dose-dependently inhibited AIA FLS proliferation in vitro.
  • Geniposide induced morphological apoptotic changes and significantly increased AIA FLS apoptosis rate.
  • Geniposide altered apoptosis-related gene expression (decreased Bcl-2, increased Bax, caspase 3) and inhibited ERK signaling pathway (reduced p-ERK1/2).

Conclusions:

  • Geniposide effectively induces apoptosis in AIA rat FLS.
  • Geniposide exerts its effects by modulating apoptosis-related gene expression and inhibiting the ERK signaling pathway.
  • Geniposide represents a potential therapeutic agent for rheumatoid arthritis by targeting FLS apoptosis.
Keywords:
ERKadjuvant-induced arthritisapoptosisfibroblast-like synoviocytesgeniposiderheumatoid arthritis

More Related Videos

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice08:43

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Published on: January 31, 2020

7.4K

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
14.0K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.4K