Cellular viability in human tumor micro-organ cultures: in situ quantitation by image processing

At present, cytotoxicity measurements using the fluorescent cytoprint assay are based on achieving complete cell death in cultures of drug-sensitive tumors. Thus, the usefulness of the assay would be extended if partial effects of chemotherapeutic drugs could be quantified. In this study, we addressed the issue by developing and validating a thresholding algorithm for automatic image processing that can be used to quantify the areas occupied by viable (i.e., fluorescent) micro-organs in the culture.