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Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
An evaluation of different steam disinfection protocols for cystic fibrosis nebulizers
K Hohenwarter1, W Prammer1, W Aichinger1
1Institute of Hygiene, Microbiology, Infectious Disease and Tropical Medicine, Klinikum Wels-Grieskirchen, 4600 Wels, Austria.
Background:
Contamination is a key element in cystic fibrosis. For this reason, nebulizer hygiene is an important, but complex and time-consuming task for cystic fibrosis patients. The aim of this study was to compare different steam disinfection and drying protocols.
Methods:
One hundred nebulizer parts were inoculated with cystic fibrosis-related bacteria in high concentrations (Burkholderia multivorans 3.9 × 10(10)/ml, Staphylococcus aureus 8.9 × 10(8/)ml and Pseudomonas aeruginosa 2.1 × 10(9)/ml). Tubes with Mycobacterium abscessus complex were additionally tested. Six steam disinfectors were compared. Different methods of drying were examined.
Results:
All tested bacteria were efficiently killed by the different steam disinfectors tested. The risk of contamination depended on the method of drying.
Conclusions:
Steam disinfection is a safe disinfection method. It is better to leave the nebulizers wet after steam disinfection than to manipulate them by active drying, which seems to be a source of recontamination.
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