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Related Experiment Video

Updated: Apr 6, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

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Label-free single-cell protein quantification using a drop-based mix-and-read system.

Alireza Abbaspourrad1, Huidan Zhang2, Ye Tao3

  • 1School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Scientific Reports
|August 4, 2015
PubMed
Summary

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This summary is machine-generated.

We developed a new assay for precise, label-free protein quantification in single cells. This high-throughput method measures absolute protein amounts without antibodies or mass spectrometry, overcoming previous technical challenges.

Area of Science:

  • Biochemistry
  • Proteomics
  • Single-cell analysis

Background:

  • Quantitative protein analysis at the single-cell level is challenging due to the minute quantities of proteins involved.
  • Existing methods often struggle with sensitivity, require complex labeling, or are not high-throughput.

Purpose of the Study:

  • To develop a novel, high-throughput assay for label-free, quantitative protein analysis in single cells.
  • To enable the measurement of absolute protein amounts in individual cells.

Main Methods:

  • Development of a mix-and-read assay utilizing drop-based microfluidics.
  • Label-free protein quantification without the need for antibodies or mass spectrometry.

Main Results:

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Related Experiment Videos

Last Updated: Apr 6, 2026

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  • Successful implementation of a high-throughput method for single-cell protein analysis.
  • Quantification of absolute protein amounts, not just relative levels.
  • Demonstration of a label-free approach overcoming common technical hurdles.

    • Conclusions:

    • The developed assay provides a significant advancement in single-cell proteomics.
    • This method offers a sensitive, high-throughput, and label-free solution for quantifying proteins in single cells.
    • Enables deeper insights into cellular heterogeneity and function through precise protein measurements.